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Yeasen Biotechnology rna quick purification kit
Rna Quick Purification Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+quick+purification+kit/gel+kit+page+preparation+quick/pmc12716907-70-8-12
Average 86 stars, based on 1 article reviews
rna quick purification kit - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Purification:

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer's instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex Real-Time PCR System (Thermo, USA). ..

Article Title: Ribosomal protein L5 induces cellular senescence via p53-p21-pRb pathway to mediate relapse of acute myeloid leukemia
Article Snippet: Immunoreactive bands were visualized by the Enchanced Chemiluminescence Kit (Thermo Fisher Scientific, #A38556) using the Fine-Do×6 Chemiluminescent Imaging system (Tanon). .. Total RNA was purified using RNA Quick purification Kit (YEASEN)and used as cDNA synthesis template for reverse transcription with the RevertAid RT kit (Thermo Fisher Scientific, #K1691). qRT-PCR was performed by LightCycle96 real-time PCR system (Roche, China) using SYBR Premix kit (Takara, #CN830S). ..

Article Title: MRPL51 expression is associated with poor prognosis and regulates lung adenocarcinoma progression via neurotensin
Article Snippet: After incubation with the second antibodies (goat anti-rabbit; 1:5,000), proteins were identified with an enhanced chemiluminescence (ECL) detection kit (Millipore, Burlington, MA, USA) in accordance with the manufacturer’s guidelines. .. Total RNA was extracted with a RNA-Quick Purification Kit (YEASEN Science, Shanghai, China) and quantified via NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Next, complement DNA (cDNA) was generated with Hifair V one-step RT-gDNA digestion SuperMix for quantitative polymerase chain reaction (qPCR) (Yeasen Biotechnology, Shanghai, China).

Article Title: Marein ameliorates the progression of osteoarthritis: An in vitro and in vivo studies.
Article Snippet: The bicinchoninic acid Protein Quantitation Assay was obtained from Thermo Fisher Scientific (Waltham, MA, USA). .. The Cell Count Kit-8, RIAP assay and RNA-Quick Purification Kit were obtained from Yeasen (Shanghai, China). .. Antibodies for iNOS (ab178945), ADAMTS-5 (ab41037), p-P65 (ab76302) and p-IkBα (ab133462) were obtained from Abcam (UK).

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis.
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer’s instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex RealTime PCR System (Thermo, USA). ..

Article Title: Machine learning identifies inflammation-related diagnostic biomarkers for primary myelofibrosis with clinical validation.
Article Snippet: .. PBMCs were isolated, and total RNA was extracted using the RNA-Quick Purification Kit (Yeasen Biotech, Shanghai). .. Complementary DNA (cDNA) was synthesized with HiScript II Q RT SuperMix for qPCR (Vazyme, Nanjing).

Article Title: Machine learning identifies inflammation-related diagnostic biomarkers for primary myelofibrosis with clinical validation
Article Snippet: .. PBMCs were isolated, and total RNA was extracted using the RNA-Quick Purification Kit (Yeasen Biotech, Shanghai). .. Complementary DNA (cDNA) was synthesized with HiScript II Q RT SuperMix for qPCR (Vazyme, Nanjing).

Article Title: Regulation and reversal of paclitaxel resistance via the STAT1-mediated apoptotic pathway in ovarian cancer
Article Snippet: .. Total RNA of cells was isolated using the RNA-Quick Purification Kit (Shanghai Yeasen Biotechnology Co., Ltd.). .. The RNA sample (500 ng) was reverse transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics) at 42°C for 1 h. RT-qPCR was carried out with BeyoFast SYBR Green RT-qPCR Mix (Beyotime Biotechnology) according to the manufacturer's protocols.

Synthesized:

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer's instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex Real-Time PCR System (Thermo, USA). ..

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis.
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer’s instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex RealTime PCR System (Thermo, USA). ..

cDNA Synthesis:

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer's instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex Real-Time PCR System (Thermo, USA). ..

Article Title: Ribosomal protein L5 induces cellular senescence via p53-p21-pRb pathway to mediate relapse of acute myeloid leukemia
Article Snippet: Immunoreactive bands were visualized by the Enchanced Chemiluminescence Kit (Thermo Fisher Scientific, #A38556) using the Fine-Do×6 Chemiluminescent Imaging system (Tanon). .. Total RNA was purified using RNA Quick purification Kit (YEASEN)and used as cDNA synthesis template for reverse transcription with the RevertAid RT kit (Thermo Fisher Scientific, #K1691). qRT-PCR was performed by LightCycle96 real-time PCR system (Roche, China) using SYBR Premix kit (Takara, #CN830S). ..

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis.
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer’s instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex RealTime PCR System (Thermo, USA). ..

Polymerase Chain Reaction:

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer's instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex Real-Time PCR System (Thermo, USA). ..

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis.
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer’s instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex RealTime PCR System (Thermo, USA). ..

Quantitative RT-PCR:

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer's instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex Real-Time PCR System (Thermo, USA). ..

Article Title: Ribosomal protein L5 induces cellular senescence via p53-p21-pRb pathway to mediate relapse of acute myeloid leukemia
Article Snippet: Immunoreactive bands were visualized by the Enchanced Chemiluminescence Kit (Thermo Fisher Scientific, #A38556) using the Fine-Do×6 Chemiluminescent Imaging system (Tanon). .. Total RNA was purified using RNA Quick purification Kit (YEASEN)and used as cDNA synthesis template for reverse transcription with the RevertAid RT kit (Thermo Fisher Scientific, #K1691). qRT-PCR was performed by LightCycle96 real-time PCR system (Roche, China) using SYBR Premix kit (Takara, #CN830S). ..

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis.
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer’s instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex RealTime PCR System (Thermo, USA). ..

Real-time Polymerase Chain Reaction:

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer's instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex Real-Time PCR System (Thermo, USA). ..

Article Title: Ribosomal protein L5 induces cellular senescence via p53-p21-pRb pathway to mediate relapse of acute myeloid leukemia
Article Snippet: Immunoreactive bands were visualized by the Enchanced Chemiluminescence Kit (Thermo Fisher Scientific, #A38556) using the Fine-Do×6 Chemiluminescent Imaging system (Tanon). .. Total RNA was purified using RNA Quick purification Kit (YEASEN)and used as cDNA synthesis template for reverse transcription with the RevertAid RT kit (Thermo Fisher Scientific, #K1691). qRT-PCR was performed by LightCycle96 real-time PCR system (Roche, China) using SYBR Premix kit (Takara, #CN830S). ..

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis.
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer’s instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex RealTime PCR System (Thermo, USA). ..

SYBR Green Assay:

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer's instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex Real-Time PCR System (Thermo, USA). ..

Article Title: Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis.
Article Snippet: After mounting on glass slides with Alcian blue staining, the thickness of the colonic mucus layer was observed under an eclipse microscope (Nikon, Tokyo, Japan). .. Total mRNA was extracted from the tissues using the RNA quick purification kit (Yeasen Biotechnology, Shanghai, China), and cDNA was synthesized using Hifair®II1st Strand cDNA Synthesis SuperMix (Yeasen Biotechnology, Shanghai, China) and GeneAmp PCR system 9700 (Applied Biosystems) following the manufacturer’s instructions. qRT-PCR was performed using Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) and QuantStudio 6 Flex RealTime PCR System (Thermo, USA). ..

Reverse Transcription:

Article Title: Ribosomal protein L5 induces cellular senescence via p53-p21-pRb pathway to mediate relapse of acute myeloid leukemia
Article Snippet: Immunoreactive bands were visualized by the Enchanced Chemiluminescence Kit (Thermo Fisher Scientific, #A38556) using the Fine-Do×6 Chemiluminescent Imaging system (Tanon). .. Total RNA was purified using RNA Quick purification Kit (YEASEN)and used as cDNA synthesis template for reverse transcription with the RevertAid RT kit (Thermo Fisher Scientific, #K1691). qRT-PCR was performed by LightCycle96 real-time PCR system (Roche, China) using SYBR Premix kit (Takara, #CN830S). ..

Spectrophotometry:

Article Title: MRPL51 expression is associated with poor prognosis and regulates lung adenocarcinoma progression via neurotensin
Article Snippet: After incubation with the second antibodies (goat anti-rabbit; 1:5,000), proteins were identified with an enhanced chemiluminescence (ECL) detection kit (Millipore, Burlington, MA, USA) in accordance with the manufacturer’s guidelines. .. Total RNA was extracted with a RNA-Quick Purification Kit (YEASEN Science, Shanghai, China) and quantified via NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Next, complement DNA (cDNA) was generated with Hifair V one-step RT-gDNA digestion SuperMix for quantitative polymerase chain reaction (qPCR) (Yeasen Biotechnology, Shanghai, China).

Cell Characterization:

Article Title: Marein ameliorates the progression of osteoarthritis: An in vitro and in vivo studies.
Article Snippet: The bicinchoninic acid Protein Quantitation Assay was obtained from Thermo Fisher Scientific (Waltham, MA, USA). .. The Cell Count Kit-8, RIAP assay and RNA-Quick Purification Kit were obtained from Yeasen (Shanghai, China). .. Antibodies for iNOS (ab178945), ADAMTS-5 (ab41037), p-P65 (ab76302) and p-IkBα (ab133462) were obtained from Abcam (UK).

Isolation:

Article Title: Machine learning identifies inflammation-related diagnostic biomarkers for primary myelofibrosis with clinical validation.
Article Snippet: .. PBMCs were isolated, and total RNA was extracted using the RNA-Quick Purification Kit (Yeasen Biotech, Shanghai). .. Complementary DNA (cDNA) was synthesized with HiScript II Q RT SuperMix for qPCR (Vazyme, Nanjing).

Article Title: Machine learning identifies inflammation-related diagnostic biomarkers for primary myelofibrosis with clinical validation
Article Snippet: .. PBMCs were isolated, and total RNA was extracted using the RNA-Quick Purification Kit (Yeasen Biotech, Shanghai). .. Complementary DNA (cDNA) was synthesized with HiScript II Q RT SuperMix for qPCR (Vazyme, Nanjing).

Article Title: Regulation and reversal of paclitaxel resistance via the STAT1-mediated apoptotic pathway in ovarian cancer
Article Snippet: .. Total RNA of cells was isolated using the RNA-Quick Purification Kit (Shanghai Yeasen Biotechnology Co., Ltd.). .. The RNA sample (500 ng) was reverse transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics) at 42°C for 1 h. RT-qPCR was carried out with BeyoFast SYBR Green RT-qPCR Mix (Beyotime Biotechnology) according to the manufacturer's protocols.



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